Last edited by Mugal
Thursday, May 7, 2020 | History

2 edition of PCR/RT-PCR in situ light and electron microscopy found in the catalog.

PCR/RT-PCR in situ light and electron microscopy

GГ©rard Morel

PCR/RT-PCR in situ light and electron microscopy

by GГ©rard Morel

  • 69 Want to read
  • 8 Currently reading

Published by CRC Press in Boca Raton, Fla .
Written in English

    Subjects:
  • Microscopy -- Technique,
  • Electron microscopy -- Technique,
  • Polymerase chain reaction,
  • Reverse transcriptase

  • Edition Notes

    StatementGérard Morel, Mireille Raccurt
    SeriesMethods of visualization
    ContributionsRaccurt, Mireille
    Classifications
    LC ClassificationsQH207 .M67 2003
    The Physical Object
    Paginationxvi, 416 p., [11] p. of plates :
    Number of Pages416
    ID Numbers
    Open LibraryOL17058583M
    ISBN 10084930041X
    LC Control Number2002073649

    An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up to , times shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects. A scanning transmission electron microscope . 1. Author(s): Morel,Gérard; Raccurt,Mireille Title(s): PCR/RT-PCR in situ light and electron microscopy/ Gérard Morel, Mireille Raccurt. Country of Publication.

    Hydroxide film was formed on Al-alloy (AlMgSi(wt.%)) sheets by steam coating with the temperature of °C for 24 h. During bending test of the coated specimens, the crack initiation and propagation processes in the hydroxide film were investigated using in situ Author: Hongmei Li, Naoki Takata, Makoto Kobashi, Ai Serizawa. It requires both high-resolution chemical analysis and microstructural identification. In situ transmission electron microscopy (TEM) coupled with electron energy loss spectroscopy (EELS) has recently Cited by:

      Here's the latest edition of the leading reference work on histological techniques. This book is an essential and invaluable resource no matter what part you play in histological preparations and applications, whether you're a student or a highly experienced laboratory professional. This is a one stop reference book /5(3).   Considering the above points, adding 1–2 cycles to the cycle number obtained from the in-solution PCR is recommended when conducting the in situ PCR. RT-PCR. Since in situ PCR is primarily used for detecting a particular mRNA, an in situ reverse transcription (RT) step precedes the in situ Cited by:


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PCR/RT-PCR in situ light and electron microscopy by GГ©rard Morel Download PDF EPUB FB2

PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy Format: Hardcover.

PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy.

PCR/RT- PCR in situ Light and Electron Microscopy PDF Free Download E-BOOK DESCRIPTION Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application.

This book covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope.

In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the BrainCited by: 1. PCR/RT-PCR in situ. LIGHT and ELECTRON MICROSCOPY Methods in Visualization Series Editor: Grard Morel In Situ Hybridization in Light Microscopy Grard Morel and Annie Cavalier Visualization of Receptors: In Situ Applications of Radioligand Binding Emmanuel Moyse and Slavica M.

Krantic Genome Visualization by Classic Methods in Light Microscopy. Biology • Free Medical Books PCR/RT- PCR in situ Light and Electron Microscopy PDF. Biology. "PCR/RT-PCR in situ Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopic.

In this protocol we describe the in situ PCR method for the amplification of both DNA and mRNA targets [in situ reverse transcriptase-PCR (RT-PCR)], from frozen or paraffin-fixed tissue Cited by: As vitrification preserves cellular structures and molecular details to the atomic level, cryo‐electron microscopy and cryo‐electron tomography offer an opportunity for in situ structural biology where.

Genre/Form: Electronic books: Additional Physical Format: Print version: Morel, Gérard. PCR/RT-PCR in situ light and electron microscopy. Boca Raton: CRC Press, © In situ PCR combines the extreme sensitivity of PCR with the cell-localizing ability of in situ hybridization.

The amplification of DNA (PCR) or a cDNA (RT-PCR) in cell or tissue sections has been developed at light and electron microscopic Cited by: New forms of imaging in science have nearly always led to major advances, especially at the nanoscale, and the pace of these developments has increased dramatically in recent decades.

Many new types of microscopes have joined the traditional light microscope and the transmission and scanning electron.

Histochemical and Cytochemical Methods of Visualization by Jean-Marie Exbrayat,available at Book Depository with free delivery : Jean-Marie Exbrayat. To this end, in situ or operando transmission electron microscopy (TEM), which is able to resolve atomic structure and elucidate proper structure-activity relationships, promises exciting opportunities for solving these challenges for advancing energy-related applications.

2 This essay focuses on the technical advances of in situ Cited by: 4. Industrial Applications Of Electron Microscopy this reference details the instrumentation and underlying principles for utilization of electron microscopy in the manufacturing, automotive, semiconductor, photographic film, pharmaceutical, chemical, mineral, forensic, glass, and pulp and paper industries.

PCR/RT- PCR in situ: Light. studied by including the time domain in electron microscopy, while taking into account the electron beam effects. CISCEM is also open to other in-situ techniques, such as light microscopy, X-ray, near field or scanning probe microscopy.

Levin Light Microscopy Techniques For Bacterial Cell Biology 3 light microscopy. In contrast to immunoelectron microscopy, which requires highly specialized expertise and equipment, IFM is Cited by: Standard light microscopes have a lateral resolution limit of about micrometers (μm) for routine analysis.

In contrast, electron microscopes have a lateral resolution of up to 1 nanometer (nm). Both living and dead specimens are viewed with a light microscope, and often in real colour, whereas only dead ones are viewed with an electron.

In situ static and dynamic light scattering and scanning electron microscopy study on the crystallization of the dense zinc imidazolate framework ZIF-zni T.

Hikov, C. Schröder, J. Cravillon, M. Wiebcke Cited by:. Two-dimensional (2D) metal sulfides have been widely explored as promising electrodes for lithium-ion batteries since their two-dimensional layered structure allows lithium ions to intercalate between Cited by: MoS 2-decorated graphitic C 3 N 4 (g-C 3 N 4 /MoS 2) photocatalysts were prepared by a simple and scalable in situ light-assisted method.

In this process, MoS 2 was formed from the reduction of [MoS 4] 2− by photogenerated electrons, and was then loaded in situ on the electron Cited by: This book highlights the current understanding of materials in the context of new and continuously emerging techniques in the field of electron microscopy.

The authors present applications of electron .